DYNAMIC.HEK T is a serum-free, chemically-defined, animal-component-free medium for transient HEK293 cells. DYNAMIC.HEK T was developed for the cultivation and expression of HEK293 and other mammalian cell lines, with a special focus on transfection applications and virus production. The medium is highly suitable for transient transfection with e.g. polycationic transfection reagents such as polyethylenimine (PEI). DYNAMIC.HEK T supports cell growth and production of recombinant proteins and antibodies in suspension culture. It can be used in research and further manufacturing.