Liquid media provide basic nutrients for all cell culture applications. Each of our high-quality cell culture media is manufactured according to the originally published formula or with modifications essential to the consistent performance and stability of the culture medium. DMEM or Dulbecco’s Modified Eagle’s Medium is a standard medium that supports the growth of various mammalian cells. It can be used to cultivate primary fibroblasts, neurons, glial cells, HUVECs, and smooth muscle cells, as well as cell lines such as HeLa, 293, Cos-7, and PC-12. The original formulation contained 1.0 g/L of glucose and was used to culture embryonic mouse cells. Since then, it has been modified in several ways. Additionally, the glucose levels have been raised to 4.5 g/L for cells with higher energy demands.