HEPES Buffer Solution (1 M)

HEPES Buffer Solution is a biological buffer used in cell culture media. Due to its stability, it is suitable for many different cell culture systems.

 Product Code



100 ml


+2 °C to +8 °C





HEPES (N-2-hydroxyethylpiperazine-N-2-ethane sulfonic acid) is a zwitterionic organic chemical buffering agent commonly used in cell culture media. It maintains the pH in a range of 6.7 – 8.6 and the addition of 10 to 25 mM HEPES provides extra buffering capacity when a cell culture requires extended periods of manipulation outside of a CO2 incubator. HEPES is suitable for many cell culture systems because it is membrane impermeable, has a limited effect on biochemical reactions, and is chemically and enzymatically stable. HEPES Buffer Solution is prepared in cell culture grade water.

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